Post-polyketide synthase steps in iso-migrastatin biosynthesis, featuring tailoring enzymes with broad substrate specificity.

نویسندگان

  • Ming Ma
  • Thomas Kwong
  • Si-Kyu Lim
  • Jianhua Ju
  • Jeremy R Lohman
  • Ben Shen
چکیده

The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) identify two nascent products of the iso-MGS AT-less type I PKS, establishing an unprecedented novel feature for AT-less type I PKSs, and (ii) account for the formation of all known post-PKS biosynthetic intermediates generated by the three tailoring enzymes MgsIJK, which possessed significant substrate promiscuities.

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عنوان ژورنال:
  • Journal of the American Chemical Society

دوره 135 7  شماره 

صفحات  -

تاریخ انتشار 2013